Jeroen den Hertog's work experience includes working at the Hubrecht Institute, Developmental Biology and Stem Cell Research as a Managing Director since January 2020, as well as a Deputy Director of Research and Groepsleider since September 1994. Jeroen has also worked at Leiden University as a Professor of Molecular Developmental Zoology since 2008 and at the Salk Institute for Biological Studies as a Postdoc from August 1992 to August 1994.

Jeroen den Hertog completed their education at Utrecht University. From 1977 to 1983, they attended WLG, where they did not earn a degree or major in any field. Next, from 1983 to 1988, they pursued a degree in Chemistry, obtaining a Drs. degree. Finally, from 1988 to 1992, they completed their education with a Doctor of Philosophy (PhD) in Developmental Biology and Embryology.

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